by VALLEJO VALLÉS, MARINA
The Python script htt17_library.py generates a library of sequences containing all the single nucleotide mutations in the Htt17 sequence. This library is necessary to perform a deep mutagenesis project. The script can be used with any other sequence in a FASTA file format.
Input files:
- htt17.fa
Output files:
-
htt17_sequences_DNA.txt
-
htt17_sequences_proteins.txt
It also prints the Hamming Distance values for each codon and the final selection.
Generated in order to continue with the Bachelor's Thesis: Mutagenesis of the Huntingtin N-terminal region to understand its role in amyloid nucleation