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Y25-012 - Changes to scRNA Core binning rules #2154

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merged 2 commits into from
Jan 10, 2025

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KatyTaylor
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@KatyTaylor KatyTaylor commented Jan 10, 2025

Closes #

Also see Integration Suite PR - https://gitlab.internal.sanger.ac.uk/psd/integration-suite/-/merge_requests/208/diffs

Changes proposed in this pull request

  • Change the diluent volume, as requested by lab staff
  • Changes to the binning ranges - requirement missed back in June

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@KatyTaylor KatyTaylor self-assigned this Jan 10, 2025
@KatyTaylor KatyTaylor linked an issue Jan 10, 2025 that may be closed by this pull request
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codecov bot commented Jan 10, 2025

Codecov Report

All modified and coverable lines are covered by tests ✅

Project coverage is 80.76%. Comparing base (4aa8737) to head (a31f222).
Report is 3 commits behind head on develop.

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@@           Coverage Diff            @@
##           develop    #2154   +/-   ##
========================================
  Coverage    80.76%   80.76%           
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  Files          477      477           
  Lines        18148    18148           
  Branches       269      269           
========================================
  Hits         14658    14658           
  Misses        3488     3488           
  Partials         2        2           
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@andrewsparkes andrewsparkes left a comment

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Looks fine. Assume Abby requested new numbers. If they need to do that often it might be a pain.

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Looks fine. Assume Abby requested new numbers. If they need to do that often it might be a pain.

Yes, there's a linked story and a conversation in Slack. Don't think they should need to do it often. For the binning ranges, Abby requested it 6 months ago but I mis-read it.

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@yoldas yoldas left a comment

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Looks good. I have checked

  • diluent volume is configured to be 25 now instead of zero.
  • per cycles are correct according to the Recommended Cycle Numbers table.
  • mid-point of Total Cycles is taken for each cDNA input.
  • for the smallest input only the max specified. OK.
  • for the biggest input only the min specified. OK.

@KatyTaylor KatyTaylor merged commit 3dbdbf0 into develop Jan 10, 2025
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@KatyTaylor KatyTaylor deleted the Y25-012-Changes-to-scRNA-Core-binning-rules branch January 10, 2025 14:26
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Y25-012 - Changes to scRNA Core binning rules
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