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# proteinsearch | ||
An automated web search for human antibodies labelling neurons and glia in the brain. Takes the direct output of Seurat's "FindMarkers()" and returns a data frame of antibodies based on information from the human protein atlas. | ||
# proteinsearch.py | ||
An automated web search for human antibodies labelling neurons and glia in the brain. | ||
This code takes the direct output of Seurat's `FindMarkers()` and returns a data frame of antibodies based on information from the human protein atlas. | ||
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## Instructions: | ||
1. Save a `.csv` of the marker genes for your single cell RNA sequencing data: `write.csv(FindMarkers(data), markers.csv)` | ||
2. In your terminal, ensure that Selenium web driver is installed: `pip install selenium` | ||
3. Download Chrome driver [here](https://chromedriver.chromium.org/downloads). | ||
4. In the terminal, navigate to the directory holding `proteinsearch.py`. | ||
5. In the terminal, type: `python proteinsearch.py` | ||
6. Enter the absolute path of your marker gene csv. | ||
7. Enter the name of of the output file (be sure to include .csv at the end). | ||
8. Chrome driver will open and begin the search! |