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Qiongjing Zou edited this page Mar 18, 2024 · 5 revisions

Welcome to the u-probe wiki!

This u-probe software package allows processing of raw ratiometric biosensor images (for example based on FRET) into fully corrected "ratio maps" or "activation maps" — images showing the localized activation of the biosensor. This includes application of all necessary image corrections needed for quantitative widefield imaging of ratiometric biosensors.

Installation

For instructions on downloading MATLAB, cloning the u-probe Git Repository, and adding the u-probe Repository to your MATLAB Path, please visit the Installation page.

Getting Started

For instructions to initialize the software in MATLAB and generate the necessary data structure for analysis, please visit the Getting Started page.

Documentation

For comprehensive descriptions of the u-probe package and explanations of parameters for each of its individual steps, please refer to the sidebar located on the right side of the Wiki page.

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