diff --git a/.changeset/itchy-ravens-train.md b/.changeset/itchy-ravens-train.md
new file mode 100644
index 00000000000..7a8905fcecd
--- /dev/null
+++ b/.changeset/itchy-ravens-train.md
@@ -0,0 +1,5 @@
+---
+"@wso2is/admin.applications.v1": patch
+---
+
+Enable request signature validation checkbox when signature validation with certificate alias is enabled
diff --git a/features/admin.applications.v1/components/forms/inbound-saml-form.tsx b/features/admin.applications.v1/components/forms/inbound-saml-form.tsx
index 1f9da659b4e..ee3783a32a6 100644
--- a/features/admin.applications.v1/components/forms/inbound-saml-form.tsx
+++ b/features/admin.applications.v1/components/forms/inbound-saml-form.tsx
@@ -813,7 +813,8 @@ export const InboundSAMLForm: FunctionComponent<InboundSAMLFormPropsInterface> =
                                             t("applications:forms.inboundSAML.sections" +
                                                 ".requestValidation.fields.signatureValidation.validations.empty")
                                         }
-                                        disabled={ !isCertAvailableForEncrypt }
+                                        disabled={ !isCertAvailableForEncrypt
+                                            && !isSignatureValidationCertificateAliasEnabled }
                                         type="checkbox"
                                         listen={
                                             (values: Map<string, FormValue>) => {