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Some difference using --kingdom option #704

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ZeWLi opened this issue Aug 30, 2024 · 0 comments
Open

Some difference using --kingdom option #704

ZeWLi opened this issue Aug 30, 2024 · 0 comments

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@ZeWLi
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ZeWLi commented Aug 30, 2024

Dear developer,

I used prokka to annotate metagenomic assembled contigs to find the ribosomal proteins. I tried --kingdom Bacteria and --kingdom Archaea options, and I noticed the .fna files were the same in 2 runs. Then I checked .tsv files, I found that some genes were annotated to different annotations (i.e. different type of ribosomal proteins). So I wonder

  1. Why this happened, and can I confidently claim the ribosomal proteins are bacterial/archaeal type?
  2. Why I also noticed the run using--kingdom Archaea generated far more ribosomal proteins than the run using --kingdom Bacteria?

Regards,
Edwin

@ZeWLi ZeWLi changed the title Does --kingdom Some difference using --kingdom option Aug 30, 2024
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