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I used prokka to annotate metagenomic assembled contigs to find the ribosomal proteins. I tried --kingdom Bacteria and --kingdom Archaea options, and I noticed the .fna files were the same in 2 runs. Then I checked .tsv files, I found that some genes were annotated to different annotations (i.e. different type of ribosomal proteins). So I wonder
Why this happened, and can I confidently claim the ribosomal proteins are bacterial/archaeal type?
Why I also noticed the run using--kingdom Archaea generated far more ribosomal proteins than the run using --kingdom Bacteria?
Regards,
Edwin
The text was updated successfully, but these errors were encountered:
ZeWLi
changed the title
Does --kingdom
Some difference using --kingdom option
Aug 30, 2024
Dear developer,
I used prokka to annotate metagenomic assembled contigs to find the ribosomal proteins. I tried
--kingdom Bacteria
and--kingdom Archaea
options, and I noticed the.fna
files were the same in 2 runs. Then I checked.tsv
files, I found that some genes were annotated to different annotations (i.e. different type of ribosomal proteins). So I wonder--kingdom Archaea
generated far more ribosomal proteins than the run using--kingdom Bacteria
?Regards,
Edwin
The text was updated successfully, but these errors were encountered: