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DPL-917 Submit plate of PBMC pools for cDNA prep #3901

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KatyTaylor opened this issue Sep 25, 2023 · 2 comments · Fixed by #4067 or sanger/limber#1657
Closed
3 tasks done

DPL-917 Submit plate of PBMC pools for cDNA prep #3901

KatyTaylor opened this issue Sep 25, 2023 · 2 comments · Fixed by #4067 or sanger/limber#1657
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@KatyTaylor
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KatyTaylor commented Sep 25, 2023

User story
As an SSR, I would like to be able to submit a plate of pooled PBMCs for cDNA prep, as part of the scRNA Core pipeline.

Who are the primary contacts for this story
Katy, Lesley, Abby

Who is the nominated tester for UAT
Lesley, Abby, SSR

Acceptance criteria
To be considered successful the solution must allow:

  • PBMC pool plates imported from faculty (output of DPL-823) can be submitted to the cDNA prep part of the scRNA Core pipeline (request type limber_scrna_core_cDNA_prep)
  • Update / create new Integration Suite test to include this submission.
  • Update documentation, to say relevant submission templates, library types etc.

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This story has a non-blocking relationship with:

Additional context
This is the second entrypoint into the pipeline - these plates are coming straight from faculty, rather than having being taken through the scRNA Core Cell Extraction pipeline in SeqOps.

@KatyTaylor
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Decision in working group meeting 2nd Nov, that these will not be combined with plates that were thawed and pooled in SeqOps.

@KatyTaylor KatyTaylor added the Size: M Medium - medium effort & risk label Nov 24, 2023
@andrewsparkes andrewsparkes self-assigned this Mar 25, 2024
@andrewsparkes andrewsparkes linked a pull request Mar 28, 2024 that will close this issue
@andrewsparkes
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andrewsparkes commented Apr 2, 2024

Gitlab branch in integration suite for 917 also made

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