Erros when downloading genewalk even with versions higher than 3.5 #63
Felipe-Leite
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Replies: 4 comments 3 replies
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Hi @Felipe-Leite, the issue seems to be with the installation of the Gensim package (https://github.com/RaRe-Technologies/gensim). Based on this page: https://ma.ttias.be/mac-os-xcrun-error-invalid-active-developer-path-missing-xcrun/, you could try running
to see if that helps with the xcode error. |
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Great, it worked.
Of notice, I used QKI_for GW list and I got several warning message like this:
WARNING: [2021-07-12 21:50:17] genewalk.gene_lists - Could not get HGNC ID for MGI ID 2686053
Otherwise, it worked very well.
Also, is there a way to make GW consider the fold change in the analyses?
Is it a problem to have long non-coding RNA, pseudogenes, etc within the input list?
Att,
Felipe de Souza Leite, PhD
Genetics and Genomics - Kunkel's lab
Boston Children's Hospital
…________________________________
Pathology - Pourquié's Lab
Brigham & Women's Hospital
______________________________
Centro de Pesquisa sobre o Genoma Humano e Células-Tronco
Universidade de São Paulo (USP)
São Paulo, Brasil.
On Jul 12, 2021, at 7:09 PM, Robert Ietswaart ***@***.***> wrote:
Hi @Felipe-Leite <https://github.com/Felipe-Leite>
This is an issue with your SSL certificate, which is a problem unrelated to GeneWalk.
Please see the workaround answered before, in case you cannot adjust your SSL certificate.
#44 (comment) <#44 (comment)>
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I’m sorry, I just notice that the warning messages are normal.
On Tue, Jul 13, 2021 at 8:35 AM Felipe de Souza Leite <
***@***.***> wrote:
… Great, it worked.
Of notice, I used QKI_for GW list and I got several warning message like
this:
WARNING: [2021-07-12 21:50:17] genewalk.gene_lists - Could not get HGNC ID
for MGI ID 2686053
Otherwise, it worked very well.
Also, is there a way to make GW consider the fold change in the analyses?
Is it a problem to have long non-coding RNA, pseudogenes, etc within the
input list?
Att,
Felipe de Souza Leite, PhD
Genetics and Genomics - Kunkel's lab
Boston Children's Hospital
________________________________
Pathology - Pourquié's Lab
Brigham & Women's Hospital
______________________________
Centro de Pesquisa sobre o Genoma Humano e Células-Tronco
Universidade de São Paulo (USP)
São Paulo, Brasil.
On Jul 12, 2021, at 7:09 PM, Robert Ietswaart ***@***.***>
wrote:
Hi @Felipe-Leite <https://github.com/Felipe-Leite>
This is an issue with your SSL certificate, which is a problem unrelated
to GeneWalk.
Please see the workaround answered before, in case you cannot adjust your
SSL certificate.
#44 (comment)
<#44 (comment)>
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You are receiving this because you were mentioned.
Reply to this email directly, view it on GitHub
<#63 (reply in thread)>,
or unsubscribe
<https://github.com/notifications/unsubscribe-auth/AEQGLJ4KFVBX3PQTXKGMIVLTXNYZTANCNFSM5AHAEQTA>
.
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Thanks, that’s great. Congratulations on the work.
Att,
Felipe de Souza Leite, PhD
Genetics and Genomics - Kunkel's lab
Boston Children's Hospital
…________________________________
Pathology - Pourquié's Lab
Brigham & Women's Hospital
______________________________
Centro de Pesquisa sobre o Genoma Humano e Células-Tronco
Universidade de São Paulo (USP)
São Paulo, Brasil.
On Jul 13, 2021, at 11:08 AM, Robert Ietswaart ***@***.***> wrote:
Hi @Felipe-Leite <https://github.com/Felipe-Leite>
Great that it works,
regarding the fold changes: GW does not take them into account, in case they are important for your analysis you can determine the input list for GW based on a fold change cut off for instance. For bulk RNAseq with enough replicates to ensure statistical power, we have used a cut off based on the DEseq p-adj values. In the end, how you decide your input list is totally up to you.
non-coding RNA and pseudogenes etc: this is fine to include, but bear in mind that these genes often do not have any GO annotations associated. In that case, GW can not make any relevance prediction. Also sometimes these genes do not have a UniProt ID associated, in that case the gene will be automatically filtered out by GW with a similar warning message as you got for MGI ID 2686053. If you are lacking statistical power with DE (not getting many DE genes), I would recommend prefiltering for protein coding genes and then run DE and base the GW input list on that. But again, this is up totally up to you.
Good luck,
Robert
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Hi! I tried many time to download Genewalk whitout success. I tried Python 3.9.6 and 3.7. Do I need a virtual environment when using updated versions?
THis is what I get:
Python 3.9.6 (v3.9.6:db3ff76da1, Jun 28 2021, 11:49:53)
[Clang 6.0 (clang-600.0.57)] on darwin
Type "help", "copyright", "credits" or "license" for more information.
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